In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. H�lU=s�8��+\B71�$DJ��M��f�k4�"vQ��Dt�8�3n�[n߮)���q�_o�~��z��Uy}SU[W��W_U�]p��f��W�����H�]Ѫ�>�m��~j�) q�oa_Fwr#T�h���-:�ǐ�����E�~�. The luminol test is a presumptive test for blood that many crime labs employ in crime scene investigation. c-�[�@�ݫ)�3 �3Z 0000001188 00000 n Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. BLUESTAR® Identi-PSA is a rapid qualitative test in a device for PSA detection from traditional biological samples (serum, plasma, or blood), but also from sperm samples collected with a swab either on the clothes or on the body of the presumed victim of a sexual assault. BlueStar Presumptive Test for Blood Published Online: March 2018 . 0000008782 00000 n regarding fish epithelial injury and outlines several presumptive tests which show promise in their ability to be used for the detection and quantification of fish epithelial injury. 0000009767 00000 n AB - Luminol, leuchomalachite green, phenolphthalein, Hemastix®, Hemident™, and Bluestar© are all used as presumptive tests for blood. The first study was performed to see how age and temperature variance would affect results for various substrates. presumptive blood test no longer in use since the reagent is a known carcinogen Luminol/Bluestar/Hemascein luminescence tests that glow in the dark in the presence of blood Leuchomalachite green had a sensitivity of 1:10,000, while the remaining tests were able to detect blood … PRESUMPTIVE BLOOD TESTING WITH BLUESTAR® FORENSIC ON 800 YEARS OLD ARCHAEOLOGICAL CONTEXTS, ANTOFAGASTA DE LA SIERRA, SOUTH HIGHLANDS (PUNA) OF ARGENTINA by Sara López Campeny y Andrés Romano Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto Superior de Estudios Sociales (ISES-CONICET) Instituto de Arqueología y Museo, Universidad Nacional … 0000017470 00000 n Tags: Question 6 . ��`��m�_���;��/u�����)e͋ Presumptive tests for blood are a common means of identifying latent, or hidden, bloodstains and may … 0000002271 00000 n ���a%Ih^h�_���(&��F�!��KDIJr�v�+��W 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case. )��� ��A C g`,u�j@l6[����CݥL��"�m�Mb,���>a`��vA���Ɓ����'@��o���^:�a�B�f��FQ�g�mژ'�3Lb\aฉK!��Ԃs�;\3����bB��q"#�� q�x8A^˘30. Recently, a new human blood confirmatory test (rapid stain identification-blood, RSID-blood) has been proposed. 0000001847 00000 n Luminol, leuchomalachite green, phenolphthalein, Hemastix s , Hemident TM , and Bluestar r are all used as presumptive tests for blood. The purpose of this study was to investigate whether using Bluestar could affect the results of the Kastle-Meyer test uses in forensic laboratories as a presumptive test. �Hy�}����$�2� H���3�9DŽjv�QJ(��]��,Ow�8� 幒��_l=��w�M�Ȩ;�Ƞ��B.���05��q�� T�aR�8:t}X78GWu�퓟$�P`��@cW�� ���ѕ��K?~�,uv���r-��oW�i����(t矫|Ĩ�w�ծ�j�o?882����rn7��#T�b��P�c�$ In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. Bluestar ® was the only presumptive test that can detect blood dilutions of 1:100,000 on some substrates and luminol was inclusive on pressure treated wood. 0000004173 00000 n Leucomalachite green (LMG) and leuko-crystal violet (LCV) are widely used for presumptive testing in casework at crime scene investigations and in the laboratories. It is critical to determine the nature of each fluid. )$�T-jؘ�x0��h!F�;��~��bڞ+��w�s�1���x~�gDxA��#��'Y2 s��B��?����TU�p���L�W b���Ʉ�5�v�[���ظ'XJ-���l'$>��̥��Ε2R���,�s�@�v� ��j)�ҏ�^}J���j'`&�Sj`w�J�Z�j�q�h=k�I���םsp��e���%�E�a��. BlueStar® and Luminol offer presumptive detection of blood but are not specific to human blood (2, 3). Critical Revision of Presumptive Tests for Bloodstains. h�bbd``b`�$��� n��?��nY�����%� e� endstream endobj startxref 0 %%EOF 90 0 obj <>stream 0000013708 00000 n 76 0 obj <> endobj 82 0 obj <>/Filter/FlateDecode/ID[<4A3F5BB85657C54CA5E709394F9662C5>]/Index[76 15]/Info 75 0 R/Length 51/Prev 296648/Root 77 0 R/Size 91/Type/XRef/W[1 2 1]>>stream h��S[o�0�+����c� 0000005526 00000 n Through the years, a number of articles have been published on the popular techniques of the day. 0000010766 00000 n Luminol, leuchomalachite green, phenolphthalein, Hemastix, Hemident, and Bluestar are all used as presumptive tests for blood. Q. %PDF-1.3 %���� Its sensitivity is such that it will evidence blood … 0000004739 00000 n H�lU�n�6��+t @mE\�MN � ���}����-f(�AQ����[RERv;3��Zޫ���8�vw���{����z� ��]����lY�5�H����'�? alternatives . 3�sy�+���.ޜ8���Ʒ]�\��ʪklm�ʏ�x=�ε������j�l���.ѝk�0�I�\���C�@#��љ��d&�g�w� � ?��Er ����];F�r 0000008570 00000 n Through the years, a number of articles have been published on the popular techniques of the day. ?��}kJ���V��f��-v� L@A}]J�g:r��xX���K�$�:"%9�Y? Blood, Presumptive Test. 0000026568 00000 n New to Sirchie's line of Blood Test Reagents! �_&��e �В�mM8ǞA�5ū��3�*�4���=rz�[3%P8V%�Sc�~�%K���]G*�rU1xב���8�%CiĂ�)f�Pm ��7�!cIh�n���uӪ�l�u��k`��� ����TT�b�[�U��ܶm��CiYe��EM�o�` ���g endstream endobj 84 0 obj 900 endobj 85 0 obj << /Filter /FlateDecode /Length 84 0 R >> stream BlueStar® and Luminol are presumptive tests for blood that crime labs use in crime scene investigations (1, 2, 3). The sample probably is the substance. FE.�n%ƻN�=�J�3�$��6�Eu��;�gs(dW)6��:��k��F�&g�s��8���kEU��6��*��Ex�"�,�xE8�o�Q�Y�WkY�r]��A��#�����y_]��irB����.�{�:���Q������@��X�� ;=4"���e �e@t 0000002863 00000 n The current presumptive blood residue testing blind study has proven that the use of Bluestar ®Forensic as a reagent is a reliable means to identify blood residue on replicated lithic artifacts. Luminol, leuchomalachite green, phenolphthalein, Hemastix, Hemident, and Bluestar are all used as presumptive tests for blood. Lipid composition was found to differ for blood samples originating from human, canine, and horse species. In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. ABSTRACT: Luminol, leuchomalachite green, phenolphthalein, Hemastix ®, Hemident ™, and Bluestar © are all used as presumptive tests for blood. Scenario: At dusk, a young man was seen riding his bicycle along the narrow, winding road that encircled a lake. Bloodstains treated with BLUESTAR® FORENSIC may be visible down to 1:10,000 dilutions. In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. 0000012710 00000 n 0000001825 00000 n 0000003420 00000 n 0000012689 00000 n 0000005505 00000 n In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. Eight different products were tested, like Betadine ® , and four reagents were considered: Tetramethylbenzidine, O-toluidine, Leuchomalachite green and BlueStar ® Forensic (BlueStar). H��TM�5�ϯ���͍hAH0BI�oڡ��j{v�?��B�=vI��9L��U�^�+ﺎQ��NÎ�~ޟ��UƉ���j The DNA loci tested were … 0000014492 00000 n The Kastle-Meyer test is applied to samples that have been subjected to chemical blood reagents in the field. The BLUESTAR Forensic Kit affords the Crime Scene Investigator a complete tool to determine the prescence or absence of blood.The extreme sensitivity of BLUESTAR allows the detection of bloodstains down to 1:10,000 dilutions, including minute traces that have been washed off, with or without detergent. trailer << /Size 104 /Info 61 0 R /Root 63 0 R /Prev 113091 /ID[<6a6f54972b734497a1a209e785289785><6a6f54972b734497a1a209e785289785>] >> startxref 0 %%EOF 63 0 obj << /Pages 59 0 R /Type /Catalog /PageLabels 57 0 R /Metadata 60 0 R >> endobj 102 0 obj << /S 336 /T 469 /L 520 /Filter /FlateDecode /Length 103 0 R >> stream Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. BLUESTAR® FORENSIC is more sensitive than other presumptive field tests for blood. Here is my groups short video presentation on how to conduct the LMG presumptive blood test. The DNA loci tested … The aim of this work was to evaluate the most effective presumptive test (with fewer false-positives) when analysing products that could look and behave like blood during a forensic screening assay. 0000001281 00000 n 62 0 obj << /Linearized 1 /O 64 /H [ 1281 566 ] /L 114459 /E 35128 /N 8 /T 113101 >> endobj xref 62 42 0000000016 00000 n 216 Blood and Blood Spatter ACTIVITY 8-1 A PRESUMPTIVE TEST FOR BLOOD Objective: By the end of this activity, you will be able to: Use the Kastle-Meyer Presumptive Blood Test to determine if a given stain contains blood. Ungraded . 0000013687 00000 n The multiplicity of information that can be derived from the thread spray MS method distinguishes it from the current presumptive Bluestar® method, by enabling the detection of hemoglobin (both α- and β-chains), the heme co-factor and lipids all from a single blood sample. A new product, the BlueStar®Latent Blood Reagent, was tested and comp ared to luminol in a series of three different studies. phenolphthalein. phenolphthalein. �r�B5����O,��iY����I��^V�B��;��ݰ��'�LL�Xz�����ch�D2%N�-�c�r�I�~�]��}��E����Y(�%D�_�H`��-����a�� 0 �O�� endstream endobj 80 0 obj << /Type /FontDescriptor /FontFile3 99 0 R /FontBBox [ 0 -208 1083 739 ] /FontName /GGBGOK+AdvEls-ent3 /Flags 32 /StemV 0 /CapHeight 566 /XHeight 700 /Ascent 696 /Descent -113 /ItalicAngle 0 /CharSet (/s/r) >> endobj 81 0 obj << /Type /Font /Encoding /WinAnsiEncoding /BaseFont /GGBGOK+AdvEls-ent3 /FirstChar 114 /LastChar 115 /Subtype /Type1 /FontDescriptor 80 0 R /Widths [ 1000 1000 ] >> endobj 82 0 obj 907 endobj 83 0 obj << /Filter /FlateDecode /Length 82 0 R >> stream u�(�5�%PuS����8߱���7��Bk:���( Presumptive blood detection tests are used by forensic investigators to detect trace amounts of blood or to investigate suspicious stains. Eight different products were tested, like Betadine ® , and four reagents were considered: Tetramethylbenzidine, O-toluidine, Leuchomalachite green and BlueStar ® Forensic (BlueStar). 0000020309 00000 n For example, the Kastle–Meyer test will show either that a sample is not blood or that the sample is probably blood but may be a less common substance. %PDF-1.6 %���� precipitin. p30. Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. Luminol, leuchomalachite green, phenolphthalein, Hemastix, Hemident, and Bluestar are all used as presumptive tests for blood. '\�� �F�5x�%ڲ#eB�8�\*��L5�n0 �{W�^$g��K٥؆�e��k��#��!%�Ŀ�6Ln�V� @�L���THv�!E[r��@�����5�K��3wNI{�?��PU�!���X��ŮIQc�fm���z(�`��M�Ɠ�x�C���:7�\�����Gc�`\�J��n�[���i;�A/d>��͙Y��� [�5/����aofXr��tp�H�߮2_w�Uo��oYaɮ&Ld�l{�|$���5�4���f\ M��:zI��+x=�uze���?�G�?ޚ�����H��RfPU�t���E�����cxOs\7{�I����>9�Gt�(�C��/���N�ܶ������'6������C�T���4#%3��Bܕ�4!�w� �:�lm3 Gɏ�����|�]5y�y{f�$�֧�R�h�����,��S�� ע�Q9 Presumptive blood detection tests are used by forensic investigators to detect trace amounts of blood or to investigate suspicious stains. SURVEY . 0000008761 00000 n J`�xK��?�������������;�^V�j��$��K��o_`�`�B˗����Xj��i��n!_����F I(�P�5�����)��5=dT��I�`ԑ��!~�\�jr�˷hJ��!݇��f��2�Ք� With the exception of one specimen, the blind test reliably indicated the presence of blood on all of the specimens which had been exposed to blood. 0000014598 00000 n However, there are several other presumptive tests for blood (Bluestar , phenolphthalein, and HemastixH) that may have benefits over the use of fluorescein, particularly for field research on wild fish. 0000008331 00000 n Scope . 300 seconds . precipitin. �2"J�� sʥ�dH��q�~�q@�a�Z���C㼦���đ%�F�?7�T�����!w�CKB�p�B �����X�R��%M�6���\c{�1l�Ox6 ��|Kőc:�ǖ7���ӧܭ+�U��\A�GBJE|�i���3z�C,#� 0000010787 00000 n Ana Castelló Ponce and Fernando A. Verdú Pascual Department of Legal Medicine College of Medicine and Odontology University of Valencia Valencia, Spain. h�b```�40�� Ā B@1f����I@!�-����-`;��7����5,l��fK���������ñd�gҌ%{�8�`l�`Ap�6�L����$�fbV�Co2�00��GNѺ �j2���iF nb- � Authority: Technical Leader ; Unofficial Copy; May Not Be Most Current Version ; Page: 1 of 3 : 1. 0000027109 00000 n The sample is definitely not a certain substance. é�ꔒ�J-�Ӽc5�b����2���P�z�����͎��ݟ�����ߴ��]5���\yM�����QYC9�C�x�T��2�%M���.Øf?�a�%4�%��d5dĬc�b�[E{�C�}.����ӏ H?��W֚��Nbe�t�����Y���ŧ���R���(I�4�^� There are many presumptive tests for blood, but these cannot provide any information about human origin of the stain detected so that every time additional confirmatory tests must be performed. A forensic investigator can be confronted with a variety of fluids at a crime or accident scene. 0000032260 00000 n 0000006499 00000 n Luminol and Bluestar . 0000007364 00000 n The DNA loci tested were D2S1338 and D19S433. A presumptive test will indicate if a biological substance such as blood is present in a stain found. The aim of this work was to evaluate the most effective presumptive test (with fewer false-positives) when analysing products that could look and behave like blood during a forensic screening assay. The aim of this study is to assess the sensitivity of RSID-blood test in old, degraded bloodstains and in some bloodstains previously treated with BlueStar Forensic, a presumptive test which is often used in crime scene investigations to detect latent bloodstains. 0000009788 00000 n 0000006945 00000 n In this case, is the 7 stain found at a crime scene is blood or not. Report an issue . 0000004540 00000 n H��T�n9��+�H͝���ی3�=������fЋ�K����"�X�� %.ｪWŋ�Ae向��vu�ێe�Ê�8Ϥ��r�IF0"��0"�ޭ��bu�+�hVV�c#�� K4U��5+�e���zD��������w�-g��Gq��T��lƤ�HI�GL����]e[��9�a��FE��63Ox96J��!4%9fLi 4X(H ]��_���"�L�=ޕ�� ��|����*�>�q����0(2W ��zTEC�����͢G!��CHA�m�e���)��ƺ��l8$�sβ�f�� In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. 0000002093 00000 n 0000011753 00000 n H�b```f``ed`c`�Z��π �@16� G������=�2��o�N�d@ Invisible stains react immediately to the BLUESTAR® FORENSIC blood reagent, provoking an intense blue color luminescence (430 nm wavelength) visible to the naked eye in darkness. Rapid Stain Identification of Human Blood (RSID ™ – Blood) is a strip test used by forensic laboratories for the detection of human blood from a variety of samples (4). The determination of whether or not a substance is blood is best made by means of a preliminary color test such as the Kastle-Meyer color test, which uses the chemical: answer choices . 0000007343 00000 n This protocol is to be used when examining an exhibit of evidence or a crime scene for the presence of blood and is only a presumptive test. , , , , , , It has recently been reported that the best overall presumptive tests were luminol and Bluestar® Forensic, because they degraded DNA to a lesser extent and Benzidine had the most critical effect on blood sample DNA, inducing degradation only hours after treatment. 0000005465 00000 n p30. Bluestar Forensic is an effective latent blood reagent used by forensic investigators in the field. Evaluation of Six Presumptive Tests for Blood, Their Specificity, Sensitivity, and Effect on High Molecular-Weight DNA ABSTRACT: Luminol, leuchomalachite green, phenolphthalein, Hemastixs, HemidentTM, and Bluestarr are all used as presumptive tests for blood. benzidine. These findings suggests that forensic science laboratory personal need to know and understand the details of how the blood was detected by the crime scene investigator and the substrate on which the blood was obtained from for … Fluorescein, a presumptive test for blood, has been shown to have the capability to detect and quantify fish epithelial injury. ��t�;]��PU/ z�8# endstream endobj 77 0 obj <>/OCGs[84 0 R 85 0 R]>>/Outlines 67 0 R/Pages 71 0 R/Type/Catalog>> endobj 78 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]>>/Rotate 0/Type/Page>> endobj 79 0 obj <>stream Chapter 3 outlines a field test which was done to compare 4 prospective presumptive tests for blood (fluorescein, Bluestar©, Hemastix® and HemidentTM) in their �$ ?��'������_X��F}�߾��q���&�1ۿR A8�����@P�s��Yls4 .j��M.���P���y(��a(����g�k��H=O�S�x���]�� ���iU�a��aԍ!��0�~u}�M3LO�aH��p �tL�����-����>�C�5 �#��� x���A~����u�%��m�'6�ʮi�6qpTuP���� )�C��m�(��Nɖ�\i��?�-� 9�ē endstream endobj 76 0 obj << /Type /Font /Encoding /WinAnsiEncoding /BaseFont /GGBGOJ+AdvHelv_R /FirstChar 44 /LastChar 121 /Subtype /Type1 /FontDescriptor 77 0 R /Widths [ 281 333 281 0 552 552 552 552 552 552 552 552 552 552 0 0 0 0 0 0 0 666 666 718 718 666 614 0 718 281 500 0 552 833 718 781 666 0 718 666 614 718 666 947 666 0 0 0 0 0 0 0 0 552 0 500 552 552 281 552 552 218 0 0 218 833 552 552 552 0 333 500 281 552 500 0 500 500 ] >> endobj 77 0 obj << /Type /FontDescriptor /FontFile3 97 0 R /FontBBox [ -156 -218 1000 770 ] /FontName /GGBGOJ+AdvHelv_R /Flags 32 /StemV 0 /CapHeight 718 /XHeight 523 /Ascent 718 /Descent -219 /ItalicAngle 0 /CharSet (/space/E/v/a/l/u/t/i/o/n/f/S/x/P/r/e/s/m/p/T/B/d/comma/h/c/y/H/g/M/hyphe\ n/W/D/N/A/one/zero/two/O/L/period/V/U/I/F/X/R/three/four/J/C/five/six/se\ ven/eight/nine) >> endobj 78 0 obj 889 endobj 79 0 obj << /Filter /FlateDecode /Length 78 0 R >> stream Presumptive tests, in medical and forensic science, analyze a sample and establish one of the following: . 0000011732 00000 n Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. 0000014703 00000 n
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